Review



anti alkaline phosphatase  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    R&D Systems anti alkaline phosphatase
    Anti Alkaline Phosphatase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/Mouse+Alkaline+Phosphatase%2FALPL+Antibody/pmc13111640-229-46-50
    Average 93 stars, based on 116 article reviews
    anti alkaline phosphatase - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor
    Article Snippet: Immunofluorescence imaging analysis was performed using a Carl Zeiss Zen 2.3 SP1 FP3 (black, v.14.0.18.201). .. The primary antibodies used for immunofluorescence and immunohistochemistry were specific for mouse LepR (1:100 dilution; catalogue number BAF497, lot number BFV1021071; R&D Systems), human LepR (1:100 dilution; ab104403, catalogue number ab104403, lot number GR3428459-3; Abcam), human GPR116/ADGRF5 (1:100 dilution; catalogue number ab111169, lot number GR48909-3; Abcam), mouse GPR116/ADGRF5 (1:100 dilution; catalogue number PA5-75810, lot number YA3799243A; Thermo Fisher), CD200 (1:100 dilution; ab33734, clone MRC OX90, lot number GR242674-4; Abcam), ACTα2/αSMA (1:100 dilution; catalogue number ab5694, lot number GR283004 ; Abcam), syndecan 4 (1:100 dilution; catalogue number SC-12766, lot number J1322; Santa Cruz Biotech), ALPL (1:100 dilution; catalogue number AF2910, lot number WYMO221121; R&D Systems), COL3α1 (1:100 dilution; catalogue number SC-271249, lot number C1723; Santa Cruz Biotech), S100A4 (1:100 dilution; catalogue number ab197896, lot number GR32313138-26; Abcam), SM22α (1:100 dilution; catalogue number ab14106, lot number GR3247382-1; Abcam), mouse FBLN2 (catalogue number PA5-21640, lot number NB3850080; Invitrogen) and human FBLN2 (catalogue number HPA001934, lot number 000008817; MilliporeSigma). .. Primary antibodies were visualized using donkey anti-rabbit IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A-31573; Invitrogen), donkey anti-rabbit IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21206; Invitrogen), donkey anti-rat IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A48272; Invitrogen), donkey anti-rat IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21208; Invitrogen), donkey anti-mouse IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21202; Invitrogen) and donkey anti-mouse IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A-31571; Invitrogen).

    Article Title: Elevated PDGF‐BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFR β Shedding from Pericytes
    Article Snippet: Brain tissue was embedded in OCT and cryosectioned coronally at 50 μm with a microtome (Cat. Microm HM 525, Thermo Fisher). .. For immunofluorescence staining, tissue sections were incubated with specific primary antibodies to mouse PDGFR β (1:100, ab32570, Abcam), CD13 (1:100, MCA2183, BIO‐RAD), CD31 (1:50, AF3628 R&D), Caveolin‐1 (1:100, 3267S, Cell signaling Technology), ALPL (1:20, AF2909, R&D), MMP14 (1;100, ab51074, Abcam), Lectin (1:200, DL‐1174, Vector), TFRC (1:100, NB100‐64979, Novus) and Albumin (ab106582, 1:100, Abcam) for 24 h at 4 °C followed by corresponding fluorescence‐linked secondary antibodies (Jackson ImmunoResearch Laboratories) for 1 to 4 h while avoiding light. .. The sections were then mounted with DAPI (8961S, Cell signaling Technology).

    Article Title: Identification and validation of the diagnostic biomarker MFAP5 for CAVD with type 2 diabetes by bioinformatics analysis
    Article Snippet: After being dried at room temperature for 20 min, frozen sections of aortic valves were fixed in 4% PFA for 30 min and then permeabilized with 0.1% Triton X-100 in PBS for another 15 min. Next, the tissues were incubated with the primary antibody (MFAP5, A23529; ABclonal, China), followed by incubation with fluorescently conjugated secondary antibody and counterstaining with 4′,6-diamidino-2-phenylindole (DAPI). .. The following antibodies were used for western blotting and immunofluorescence: RUNX2 (CST, 8486), ALPL (R&D, MAB1448), GAPDH (Proteintech, 60004-1-Ig), MFAP5 (Proteintech, 15727-1-AP), goat anti-rabbit IgG (ab150077, 1:200 dilution), goat anti-mouse IgG (ab150115, 1:200 dilution). .. Experimental data were analyzed using GraphPad Prism 9 (GraphPad Software, Inc., CA, USA).

    Immunohistochemistry:

    Article Title: Prevention and treatment of peri-implant fibrosis by functionally inhibiting skeletal cells expressing the leptin receptor
    Article Snippet: Immunofluorescence imaging analysis was performed using a Carl Zeiss Zen 2.3 SP1 FP3 (black, v.14.0.18.201). .. The primary antibodies used for immunofluorescence and immunohistochemistry were specific for mouse LepR (1:100 dilution; catalogue number BAF497, lot number BFV1021071; R&D Systems), human LepR (1:100 dilution; ab104403, catalogue number ab104403, lot number GR3428459-3; Abcam), human GPR116/ADGRF5 (1:100 dilution; catalogue number ab111169, lot number GR48909-3; Abcam), mouse GPR116/ADGRF5 (1:100 dilution; catalogue number PA5-75810, lot number YA3799243A; Thermo Fisher), CD200 (1:100 dilution; ab33734, clone MRC OX90, lot number GR242674-4; Abcam), ACTα2/αSMA (1:100 dilution; catalogue number ab5694, lot number GR283004 ; Abcam), syndecan 4 (1:100 dilution; catalogue number SC-12766, lot number J1322; Santa Cruz Biotech), ALPL (1:100 dilution; catalogue number AF2910, lot number WYMO221121; R&D Systems), COL3α1 (1:100 dilution; catalogue number SC-271249, lot number C1723; Santa Cruz Biotech), S100A4 (1:100 dilution; catalogue number ab197896, lot number GR32313138-26; Abcam), SM22α (1:100 dilution; catalogue number ab14106, lot number GR3247382-1; Abcam), mouse FBLN2 (catalogue number PA5-21640, lot number NB3850080; Invitrogen) and human FBLN2 (catalogue number HPA001934, lot number 000008817; MilliporeSigma). .. Primary antibodies were visualized using donkey anti-rabbit IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A-31573; Invitrogen), donkey anti-rabbit IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21206; Invitrogen), donkey anti-rat IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A48272; Invitrogen), donkey anti-rat IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21208; Invitrogen), donkey anti-mouse IgG Alexa Fluor 488 (1:2,000 dilution; catalogue number A-21202; Invitrogen) and donkey anti-mouse IgG Alexa Fluor 647 (1:2,000 dilution; catalogue number A-31571; Invitrogen).

    Staining:

    Article Title: Elevated PDGF‐BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFR β Shedding from Pericytes
    Article Snippet: Brain tissue was embedded in OCT and cryosectioned coronally at 50 μm with a microtome (Cat. Microm HM 525, Thermo Fisher). .. For immunofluorescence staining, tissue sections were incubated with specific primary antibodies to mouse PDGFR β (1:100, ab32570, Abcam), CD13 (1:100, MCA2183, BIO‐RAD), CD31 (1:50, AF3628 R&D), Caveolin‐1 (1:100, 3267S, Cell signaling Technology), ALPL (1:20, AF2909, R&D), MMP14 (1;100, ab51074, Abcam), Lectin (1:200, DL‐1174, Vector), TFRC (1:100, NB100‐64979, Novus) and Albumin (ab106582, 1:100, Abcam) for 24 h at 4 °C followed by corresponding fluorescence‐linked secondary antibodies (Jackson ImmunoResearch Laboratories) for 1 to 4 h while avoiding light. .. The sections were then mounted with DAPI (8961S, Cell signaling Technology).

    Incubation:

    Article Title: Elevated PDGF‐BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFR β Shedding from Pericytes
    Article Snippet: Brain tissue was embedded in OCT and cryosectioned coronally at 50 μm with a microtome (Cat. Microm HM 525, Thermo Fisher). .. For immunofluorescence staining, tissue sections were incubated with specific primary antibodies to mouse PDGFR β (1:100, ab32570, Abcam), CD13 (1:100, MCA2183, BIO‐RAD), CD31 (1:50, AF3628 R&D), Caveolin‐1 (1:100, 3267S, Cell signaling Technology), ALPL (1:20, AF2909, R&D), MMP14 (1;100, ab51074, Abcam), Lectin (1:200, DL‐1174, Vector), TFRC (1:100, NB100‐64979, Novus) and Albumin (ab106582, 1:100, Abcam) for 24 h at 4 °C followed by corresponding fluorescence‐linked secondary antibodies (Jackson ImmunoResearch Laboratories) for 1 to 4 h while avoiding light. .. The sections were then mounted with DAPI (8961S, Cell signaling Technology).

    Fluorescence:

    Article Title: Elevated PDGF‐BB from Bone Impairs Hippocampal Vasculature by Inducing PDGFR β Shedding from Pericytes
    Article Snippet: Brain tissue was embedded in OCT and cryosectioned coronally at 50 μm with a microtome (Cat. Microm HM 525, Thermo Fisher). .. For immunofluorescence staining, tissue sections were incubated with specific primary antibodies to mouse PDGFR β (1:100, ab32570, Abcam), CD13 (1:100, MCA2183, BIO‐RAD), CD31 (1:50, AF3628 R&D), Caveolin‐1 (1:100, 3267S, Cell signaling Technology), ALPL (1:20, AF2909, R&D), MMP14 (1;100, ab51074, Abcam), Lectin (1:200, DL‐1174, Vector), TFRC (1:100, NB100‐64979, Novus) and Albumin (ab106582, 1:100, Abcam) for 24 h at 4 °C followed by corresponding fluorescence‐linked secondary antibodies (Jackson ImmunoResearch Laboratories) for 1 to 4 h while avoiding light. .. The sections were then mounted with DAPI (8961S, Cell signaling Technology).

    Western Blot:

    Article Title: Identification and validation of the diagnostic biomarker MFAP5 for CAVD with type 2 diabetes by bioinformatics analysis
    Article Snippet: After being dried at room temperature for 20 min, frozen sections of aortic valves were fixed in 4% PFA for 30 min and then permeabilized with 0.1% Triton X-100 in PBS for another 15 min. Next, the tissues were incubated with the primary antibody (MFAP5, A23529; ABclonal, China), followed by incubation with fluorescently conjugated secondary antibody and counterstaining with 4′,6-diamidino-2-phenylindole (DAPI). .. The following antibodies were used for western blotting and immunofluorescence: RUNX2 (CST, 8486), ALPL (R&D, MAB1448), GAPDH (Proteintech, 60004-1-Ig), MFAP5 (Proteintech, 15727-1-AP), goat anti-rabbit IgG (ab150077, 1:200 dilution), goat anti-mouse IgG (ab150115, 1:200 dilution). .. Experimental data were analyzed using GraphPad Prism 9 (GraphPad Software, Inc., CA, USA).



    Similar Products

    99
    Thermo Fisher gene exp alpl hs01029144 m1
    Gene Exp Alpl Hs01029144 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/Gene+Exp%2E+ALPL%2C+Hs01029144_m1/pm42041566-72-14--1
    Average 99 stars, based on 1 article reviews
    gene exp alpl hs01029144 m1 - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    93
    R&D Systems anti alkaline phosphatase
    Anti Alkaline Phosphatase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/Mouse+Alkaline+Phosphatase%2FALPL+Antibody/pmc13111640-229-46-50
    Average 93 stars, based on 1 article reviews
    anti alkaline phosphatase - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    86
    Bio Basic Canada tnap variants full length mouse alpl
    Tnap Variants Full Length Mouse Alpl, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/alpl+full+length+mouse+tnap+variants/pm42020733-564-5-13
    Average 86 stars, based on 1 article reviews
    tnap variants full length mouse alpl - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    94
    R&D Systems alp
    Alp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/Human+Alkaline+Phosphatase%2FALPL+Antibody/pm41961374-109-25-26
    Average 94 stars, based on 1 article reviews
    alp - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    93
    R&D Systems mouse polyclonal antibody against alkaline phosphatase
    Mouse Polyclonal Antibody Against Alkaline Phosphatase, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alpl/Mouse+Alkaline+Phosphatase%2FALPL+Antibody/pm41916075-31-17-23
    Average 93 stars, based on 1 article reviews
    mouse polyclonal antibody against alkaline phosphatase - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    Image Search Results